Epigenetic ant reprogramming

Epigenetics is an exciting but a weird area. It’s well recognized that messing with chromatin and chemical modifications of nucleic acids has profound consequences at the cellular and organism levels. But for me the mechanistic rationale for targeting epigenome pharmacologically was always somewhere close to throwing a monkey wrench into the clockworks and watching what will happen. It seems (not surprisingly) that in fact the effects are more predictable.

A recent paper in Science describes a collaborative work of three labs studying epigenetic manipulation of ants’ behavior. So the first thought in my head was ‘why the heck ants?’ I found the graphic answer at the home page of Reinberg lab (one of the corresponding authors).

[…] studying the establishment and maintenance of individual identity in the ant colony may provide us with a new angle to study the establishment and maintenance of cell identity in multi-cellular organisms such as ourselves. This is not as far fetched as it may seem; after all, much that we know about the molecular mechanisms of embryonic development in humans was learned from studies of the humble fruit fly, Drosophila melanogaster. Source

Well, the analogy between ants in a colony and cells in a multicellular organism indeed seems quite far fetched. First of all because ants are themselves multicellular organisms, so Prof. Danny Reinberg’s explanation is somewhat recursive.

Another group of authors (Jürgen Liebig lab) started long ago from chemical signaling of ants and eventually ended up in ants’ epigenetics. The third lab (Shelley L. Berger) studies epigenetics in a broad spectrum of models, ants being one of them.

But enough prelude, let’s take a look at the actual science. The most exciting part of the paper is of course the concept: by injecting some chemicals in young ants brains (or by feeding!) authors could make them swap their social roles in adulthood. Sounds so antiutopic, doesn’t it?

The whole study is built around modulation of histone diacetylases (HDACs). It appears that 10-hydroxy-2-decenoic acid (10-HDA, a.k.a. queen bee acid), a component of royal jelly made by queen bees, is a HDAC inhibitor. The acid helps keeping subordination in bee colonies. So authors decided to check if there’s similar mechanism involved in homeostasis of ant colonies.

First, the authors observed a whole bunch of ant colonies to confirm that minor workers (with small size) are main foragers, while major (big) workers do not bother very much to bring food to the colony. Then they found correlation between the foraging activity and mRNA level of two HDACs in ant brains. Then inhibited HDAC with valproic acid (VPA) and trichostatin (TSA) and found that foraging activity of minors increased. And after that they inhibited CREB binding protein (CBP), an enzyme with opposite to HDACs acitivity, by a small molecule C646, and showed that it reversed behavioral consequences of HDAC inhibition and by itself it actually completely stopped foraging activity of minors, making them major-like.


After that authors performed the same battery of pharmacological tests with majors, adding RNAi to that, and could make them to behave minor-like (i.e. to forage).

To figure out what’s going on at the level of individual genes, the authors performed series of genomic analyses: whole-brain RNA-seq and ChIP-seq. They found out that a number of neuron-regulating genes was altered in different manner upon treatment with either TSA or C646 (along with bunch of other genes apparently, but the functions of those are not easily paralleled with behavioral plasticity).

As a result, we have a very strong case that epigenetic regulation has a very pronounced (and non-lethal) effect on the behavior of social insects. But on the molecular level it still remains a complex black box. Add to this time-dependence of the effects observed in this study and you’ll agree that it’s still difficult to predict the outcomes of external epigenetic modulation. Phenotypic screening and whole-organism studies are probably the best ways to explore it.


Author: Slava Bernat

I did my PhD in medicinal chemistry/chemical biology of G protein-coupled receptors and then explored some chemical biology of non-coding RNA as a postdoc. Currently I'm working in a small biotech company in San-Francisco Bay area as a research chemist. I'm writing about science, which catches my attention in rss feed reader and some random thoughts or tutorials.

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s


profiting from randomness

R-statistics blog

Statistics with R, and open source stuff (software, data, community)

Colorblind Chemistry

The blog of Marshall Brennan, PhD

ACS Careers Blog

Career advice from the American Chemical Society

Lab Without Benches

Career skills for scientists


forcing molecules to behave


mostly science

Org Prep Daily

synthetic procedures I tried and liked

Sussex Drug Discovery Centre

Medicinal, Chemistry and Biochemistry blog from the Sussex Drug Discovery Centre

Practical Fragments

mostly science

Chemical connections

...chemistry & other curiosities

Just Like Cooking

mostly science

%d bloggers like this: